Direct electrical detection of hybridization at DNA-modified silicon surfaces

TitleDirect electrical detection of hybridization at DNA-modified silicon surfaces
Publication TypeJournal Article
Year of Publication2004
AuthorsCai, W, Peck, JR, van der Weide, DW, Hamers, RJ
JournalBiosensors & Bioelectronics
Volume19
Pagination1013-1019
Date PublishedApr
Type of ArticleArticle
ISBN Number0956-5663
Accession NumberISI:000220276100008
Keywordssilicon
Abstract

Electrochemical impedance spectroscopy was used to investigate the changes in interfacial electrical properties that arise when DNA-modified Si(1 1 1) surfaces are exposed to soution-phase DNA oligonucleotides with complementary and non-complementary sequences. The n- and p-type silicon(1 1 1) samples were covalently linked to DNA molecules via direct Si-C linkages without any intervening oxide layer. Exposure to solutions containing DNA oligonucleotides with the complementary sequence produced significant changes in both real and imaginary components of the electrical impedance, while exposure to DNA with non-complementary sequences generated negligible responses. These changes in electrical properties were corroborated with fluorescence measurements and were reproduced in multiple hybridization-denaturation cycles. The ability to detect DNA hybridization is strongly frequency-dependent. Modeling of the response and comparison of results on different silicon bulk doping shows that the sensitivity to DNA hybridization arises from DNA-induced changes in the resistance of the silicon substrate and the resistance of the molecular layers. (C) 2003 Elsevier B.V. All rights reserved.

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